Which of the statements below regarding the methods of Henry for AST and ALT is correct?

The statement that hemolysis will cause positive interference in both AST and ALT assays is accurate because hemolysis releases intracellular components from red blood cells that can artificially elevate the enzyme levels measured in the serum. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are both enzymes found in high concentrations in liver and muscle tissues. When hemolysis occurs, it can release these enzymes, leading to falsely elevated results that do not accurately reflect the patient's true enzyme levels in the non-hemolyzed serum. This characteristic of AST and ALT assays emphasizes the importance of sample handling and processing to minimize hemolysis and ensure the validity of the results.

In contrast, frozen samples at -20°C typically maintain enzyme activity for a certain duration, although prolonged storage and repeated freeze-thaw cycles can lead to a loss of activity, meaning that care must be taken with handling, but this does not inherently apply to all situations. The absorbance measurement at the start of the reaction being above a specific value, like 1.0 A, could indicate a too-concentrated analysis that could lead to non-linear readings in spectrophotometry, but this does not apply as universally as the impact of hemolysis