Which method uses the coupling enzyme G-6-PD in glucose measurement?

The hexokinase method utilizes the enzyme glucose-6-phosphate dehydrogenase (G-6-PD) in glucose measurement. In this method, glucose is first phosphorylated to glucose-6-phosphate by the action of hexokinase, using ATP as a phosphate donor. G-6-PD then catalyzes the oxidation of glucose-6-phosphate to 6-phosphogluconate while reducing NADP+ to NADPH. The increase in NADPH can be quantitatively measured, which correlates directly with the glucose concentration in the sample. This method is known for its high specificity and sensitivity.

The other methods do not involve G-6-PD as a critical component in their procedures. For instance, the Trinder glucose method uses a colorimetric reaction that does not rely on G-6-PD, while the polarographic and amperometric methods are generally electrochemical techniques that measure glucose without employing this specific enzymatic coupling.