Which hemoglobin separates from Hgb S on citrate agar, but not on agarose or cellulose acetate?

The hemoglobin that separates from Hgb S on citrate agar, but not on agarose or cellulose acetate, is Hgb DPunjab. This is significant because Hgb DPunjab is known to have similar electrophoretic mobility to Hgb S under certain conditions. When utilizing citrate agar, the differences in charge and structure can lead to Hgb DPunjab migrating differently than Hgb S, allowing for their separation.

Electrophoresis on agarose and cellulose acetate, however, might not provide the same separation due to the nature of the buffer systems used and their effects on the migration of hemoglobins. Hgb DPunjab often has a slightly different charge that can be more easily distinguished on citrate agar compared to the other methods, but under the conditions of agarose and cellulose acetate, the separation may not be evident, making Hgb DPunjab the correct choice in this situation.

The other hemoglobins mentioned, such as Hgb E, Hgb CHarlem (Georgetown), and Hgb OArab, have distinct electrophoretic properties that do not align with Hgb S in the same manner as Hgb DPunjab does on citrate agar, which is crucial for understanding their separation characteristics.