Which enzyme is used to measure plasma or serum salicylate?

The correct choice for measuring plasma or serum salicylate is salicylate hydroxylase. This enzyme catalyzes the oxidative transformation of salicylate, which allows for the quantification of salicylate levels in the blood. Salicylate hydroxylase facilitates a specific reaction that is highly relevant for monitoring and understanding salicylate toxicity, particularly since salicylate is the active metabolite of aspirin.

Using salicylate hydroxylase for this purpose provides a reliable and effective method for the clinical measurement of salicylate concentrations, helping clinicians assess the risk of toxicity and make informed decisions regarding treatment.

Other enzymes mentioned have different roles or are not specific to salicylate measurement. Peroxidase, for instance, is predominantly involved in the breakdown of hydrogen peroxide and does not relate to salicylate. Salicylate esterase acts on ester bonds and is not typically used for directly measuring salicylate levels. p-Aminosalicylate oxidase is involved in the metabolism of p-aminosalicylic acid, which does not provide insight into salicylate concentrations in serum or plasma. The specificity and catalytic efficiency of salicylate hydroxyl