What is the correct order of Hgb migration on agarose or cellulose acetate at pH 8.6?

The order of hemoglobin (Hgb) migration on agarose or cellulose acetate at pH 8.6 is determined by the net charge of the different hemoglobin variants. At this alkaline pH, hemoglobin variants will migrate according to their isoelectric points, with more negatively charged variants moving further towards the anode.

In this context, "C" represents Hemoglobin C, "S" is Hemoglobin S, "F" refers to Hemoglobin F, and "A" denotes Hemoglobin A. At pH 8.6:

1. Hemoglobin A is the most negatively charged and will migrate the farthest towards the anode.

2. Hemoglobin S has a slightly lower charge compared to Hemoglobin A, so it migrates next.

3. Hemoglobin C is less negatively charged than both Hemoglobin A and S, thus it migrates behind them.

4. Finally, Hemoglobin F, which has a charge that is less negative compared to the others, will migrate the least and stay closest to the origin.

This specific order of migration aligns with the correct answer. Therefore, Hemoglobin C will migrate ahead of S, followed by A, and then F, resulting in the order of C → S