The polarographic method for glucose measurement is based on which principle?

The polarographic method for glucose measurement relies on the principle of electrochemical detection, specifically focusing on the change in electrical potential that occurs as glucose is oxidized. In this method, glucose undergoes an oxidation reaction, which in turn involves the transfer of electrons. As glucose is oxidized, it generates an electric current that is proportional to the concentration of glucose in the solution.

While the rate of O2 depletion is an important factor in certain types of polarographic methods, the primary principle driving the glucose measurement directly relates to the change in electrical potential as glucose is being oxidized. This allows for a direct correlation between the measured current and the concentration of glucose, providing an effective means for quantitative analysis.

Other principles mentioned, such as nonenzymatic oxidation of glucose or chemiluminescence caused by ATP formation, are not foundational to the polarographic approach. The method's core is anchored in the relationship between redox reactions and the resulting electrical signals, making the change in electrical potential during the oxidation of glucose the determining principle for accurate measurement.