In the Oliver-Rosalki method, what enzyme(s) is used in the coupling reactions to measure CK activity?

The Oliver-Rosalki method is a well-established technique used to measure creatine kinase (CK) activity. This method involves a coupling reaction that relies on specific enzymes to facilitate the conversion of substrates into measurable products. In this method, hexokinase plays a critical role by catalyzing the phosphorylation of glucose to glucose-6-phosphate, while glucose-6-phosphate dehydrogenase (G-6-PD) assists in the oxidation of glucose-6-phosphate to produce NADPH from NADP+. The formation of NADPH can be observed spectrophotometrically, allowing for the quantification of CK activity based on the extent of the reaction.

This coupling of hexokinase and G-6-PD is crucial because it provides a linked enzymatic pathway that ensures the measurement of CK activity is both sensitive and specific. Thus, the correct answer emphasizes the importance of these particular enzymes in the accurate assessment of CK levels in clinical samples.