In peroxidase-coupled glucose methods, which reagent interacts with the chromogen during the coupling step?

In peroxidase-coupled glucose methods, phenol interacts with the chromogen during the coupling step. This process typically involves the oxidation of glucose, which is catalyzed by glucose oxidase. The produced hydrogen peroxide is then utilized by peroxidase to oxidize phenol, generating a colored product known as a chromogen.

Phenol is a crucial reagent in this context because, when oxidized, it contributes to the formation of the chromogenic compound, which can be measured spectrophotometrically. This measurement provides a quantitative assessment of glucose concentration in the sample.

The interactions of other reagents, such as nitroprusside, tartrate, or hydroxide, do not play the same role in this specific peroxidase-coupled method. Nitroprusside is often associated with detecting other substances, tartrate serves as a buffer or stabilizing agent, and hydroxide could affect pH levels but is not directly involved in the coupling step for chromogen formation in this context.